Anti-Cathelicidin antibody
| Name | Anti-Cathelicidin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72012 |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ELISA FC |
| Species Reactivities | Human |
| Antigen | A synthetic peptide from part of human Cathelicidin, conjugated to an immunogenic carrier protein |
| Description | Sheep Polyclonal |
| Gene | CAMP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with ab72012, diluted 50µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
Lane 1 : Anti-Cathelicidin antibody (ab72012) at 50 µg/mlLane 2 : Anti-Cathelicidin antibody (ab72012) at 50 µg/ml (pre-absorbed with immunising peptide)Lane 1 : Whole cell lysate prepared from human neutrophilsLane 2 : Whole cell lysate prepared from human neutrophils