Anti-Cathepsin L antibody
| Name | Anti-Cathepsin L antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58991 |
| Prices | $388.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Electron microscopy WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, C. elegans |
| Antigen | Synthetic peptide derived from mature region of Cathepsin L (Caenorhabditis elegans) |
| Description | Rabbit Polyclonal |
| Gene | CTSL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Cathepsin L antibody (ab58991) at 1/500 dilution + Whole cell lysate prepared from human HCT116 cell at 10 µgSecondaryHRP-conjugated donkey ppolyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab58991 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58991, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.