Anti-CaV1.3 antibody [S38-8]
| Name | Anti-CaV1.3 antibody [S38-8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab84811 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S38-8 |
| Applications | IP ICC/IF ICC/IF WB Agglutination |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Fusion protein corresponding to C terminal amino acids 2025-2161 of Rat Cav1 |
| Description | Mouse Monoclonal |
| Gene | CACNA1D |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
![All lanes : Anti-CaV1.3 antibody [S38-8] (ab84811) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Rat tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21753_CaV13-Primary-antibodies-ab84811-2.jpg)
All lanes : Anti-CaV1.3 antibody [S38-8] (ab84811) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Rat tissue lysate
![Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21754_CaV13-Primary-antibodies-ab84811-24.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.