Anti-Caveolin-2 antibody [EPR5471]

• Supplier: Abcam
• Catalog: ab133484
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5471
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2 (C terminal)
• Description: Rabbit Monoclonal
• Gene: CAV2
• Conjugate: Unconjugated

Product images

ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilutionLane 1 : HUVEC cell lysateLane 2 : HeLa cell lysateLane 3 : 3T3-L1 cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution