Anti-CD14 antibody [ EPR3652]

• Supplier: Abcam
• Catalog: ab133503
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3652
• Applications: ICC/IF FC IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to amino acid residues in the extracellular domain of Human CD14
• Description: Rabbit Monoclonal
• Gene: CD14
• Conjugate: Unconjugated

Product images

Human peripheral blood cells stained with ab133503 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab133503, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling CD14 with ab133503 at 1/100 dilution.

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD14 with ab133503 at 1/100 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD