All lanes : Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilutionLane 1 : K562 cell extracts treated with PMA (125ng/ml, 30mins)Lane 2 : K562 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
IHC image of CD32B staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74092, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.