Anti-CD35 antibody [E11]
| Name | Anti-CD35 antibody [E11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab25 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | E11 |
| Applications | WB FC IHC-P IP IHC-F |
| Species Reactivities | Human, Monkey, Monkey, Monkey |
| Antigen | Tissue, cells or virus corresponding to CD35 |
| Description | Mouse Monoclonal |
| Gene | CR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab25 (1µg/ml) staining CD35 in human spleen.Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Using mutagenesis and structural biology to map the binding site for the - Using mutagenesis and structural biology to map the binding site for the
Park HJ, Guariento M, Maciejewski M, Hauhart R, Tham WH, Cowman AF, Schmidt CQ, Mertens HD, Liszewski MK, Hourcade DE, Barlow PN, Atkinson JP. J Biol Chem. 2014 Jan 3;289(1):450-63.
Optimization of total protein and activity assays for the detection of MMP-12 in - Optimization of total protein and activity assays for the detection of MMP-12 in
LaPan P, Brady J, Grierson C, Fleming M, Miller D, Sypek J, Fu B. BMC Pulm Med. 2010 Aug 2;10:40.
Identification of an anti-monocyte monoclonal antibody that is specific for - Identification of an anti-monocyte monoclonal antibody that is specific for
Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ. Eur J Immunol. 1984 Mar;14(3):236-43.