Anti-CD40 antibody

• Supplier: Abcam
• Catalog: ab58391
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB ELISA IHC-P
• Species Reactivities: Human, Mouse
• Antigen: Synthetic peptide derived from the C-terminal of CD40 (Human)
• Description: Rabbit Polyclonal
• Gene: CD40
• Conjugate: Unconjugated

Product images

All lanes : Anti-CD40 antibody (ab58391) at 1/500 dilutionLane 1 : COS7 cell extract with no immunizing peptideLane 2 : COS7 cell extract with immunizing peptide

ab58391, at 1/50 diltuion, staining human CD40 in breast carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin embedded tissue.

ICC/IF image of ab58391 stained RAW246.7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

T cells stimulate catabolic gene expression by the stromal cells from giant cell - T cells stimulate catabolic gene expression by the stromal cells from giant cell

Cowan RW, Ghert M, Singh G. Biochem Biophys Res Commun. 2012 Mar 23;419(4):719-23. doi:

Neutralization of granulocyte macrophage colony-stimulating factor decreases - Neutralization of granulocyte macrophage colony-stimulating factor decreases

Manczak M, Mao P, Nakamura K, Bebbington C, Park B, Reddy PH. Hum Mol Genet. 2009 Oct 15;18(20):3876-93.