Anti-CD73 antibody [EPR6115]

• Supplier: Abcam
• Catalog: ab124725
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6115
• Applications: WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD73 aa 500 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: NT5E
• Conjugate: Unconjugated

Product images

All lanes : Anti-CD73 antibody [EPR6115] (ab124725) at 1/2000 dilution (purified)Lane 1 : A375 cell lysateLane 2 : Human spleen tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-CD73 antibody [EPR6115] (ab124725) at 1/1000 dilution (unpurified)Lane 1 : A375 cell lysateLane 2 : Human spleen tissue lystateLane 3 : Human fetal liver tissue lysateLysates/proteins at 10 µg per lane.SecondaryLanes 1 & 3 : HRP-conjugated goat anti-rabbit IgG at 1/200 dilutionLane 2 : HRP-conjugate goat anti-rabbit IgG at 1/200 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD73 with purified ab124725 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD73 with unpurified ab124725 at a 1/100 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD