Anti-CD79a antibody [HM47/A9]
| Name | Anti-CD79a antibody [HM47/A9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3121 |
| Prices | $400.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | HM47/A9 |
| Applications | ICC/IF ICC/IF IHC-P WB FC |
| Species Reactivities | Mouse, Rat, Bovine, Human, Pig, Monkey, Rabbit, Horse, Chicken, Guinea Pig |
| Antigen | Synthetic peptide: GTYQDVGSLNIADVQ , corresponding to C terminal amino acids 208-222 of Human CD79a |
| Description | Mouse Monoclonal |
| Gene | CD79A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Human peripheral blood lymphocytes stained with ab3121 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab3121, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25722_ab3121-4-ab3121FC.jpg)
Human peripheral blood lymphocytes stained with ab3121 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab3121, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral
ab3121 at 1/200 staining rat lymphoid tissues sections (Spleen, Lymph Node, Payers Patch) by IHC-P. The tissue was formaldehyde fixed and blocked before incubation with the antibody for 1 hour. A rabbit anti-mouse antibody was used as the secondary.See Abreview
ab3121 staining human tonsil sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h . A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.See Abreview
ab3121 staining monkey spleen sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.See Abreview
Product References
POZ-, AT-hook-, and zinc finger-containing protein (PATZ) interacts with human - POZ-, AT-hook-, and zinc finger-containing protein (PATZ) interacts with human
Pero R, Palmieri D, Angrisano T, Valentino T, Federico A, Franco R, Lembo F, Klein-Szanto AJ, Del Vecchio L, Montanaro D, Keller S, Arra C, Papadopoulou V, Wagner SD, Croce CM, Fusco A, Chiariotti L, Fedele M. J Biol Chem. 2012 May 25;287(22):18308-17.
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast - Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast
Drukker M, Tang C, Ardehali R, Rinkevich Y, Seita J, Lee AS, Mosley AR, Weissman IL, Soen Y. Nat Biotechnol. 2012 May 27;30(6):531-42.
Variation in characteristics of islets of Langerhans in insulin-resistant, - Variation in characteristics of islets of Langerhans in insulin-resistant,
Jones HB, Nugent D, Jenkins R. Int J Exp Pathol. 2010 Jun;91(3):288-301.
The role of intrapulmonary de novo lymphoid tissue in obliterative bronchiolitis - The role of intrapulmonary de novo lymphoid tissue in obliterative bronchiolitis
Sato M, Hirayama S, Hwang DM, Lara-Guerra H, Wagnetz D, Waddell TK, Liu M, Keshavjee S. J Immunol. 2009 Jun 1;182(11):7307-16.