Anti-CDC42 antibody [M152]

• Supplier: Abcam
• Catalog: ab41429
• Prices: $392.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: M152
• Applications: ICC/IF ICC/IF IHC-F WB ELISA
• Species Reactivities: Mouse, Rat, Rabbit, Human
• Antigen: Recombinant CDC42 (Rat)
• Description: Mouse Monoclonal
• Gene: CDC42
• Conjugate: Unconjugated

Product images

Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilutionLanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilutionLane 1 : human jurkat cellsLane 2 : human jurkat cellsLane 3 : mouse brainLane 4 : mouse brainLysates/proteins at 20 µg per lane.

ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.

Product References

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