Anti-CDC5L antibody [2136C1a]
| Name | Anti-CDC5L antibody [2136C1a] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51320 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2136C1a |
| Applications | FC WB IP ICC/IF DB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant human CDC5L fragment |
| Description | Mouse Monoclonal |
| Gene | CDC5L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + HeLa whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26469_ab51320wb1.gif)
Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + HeLa whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique
ab51320 at 1/10 dilution staining human CDC5L in HeLa cells by Immunocytochemistry. 1/200 dilution AlexaFluor488 Goat Anti-mouse IgG was used as a secondary antibody.
![Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26471_ab51320wb2.gif)
Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique
Immunoprecipitation analysis using ab51320 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uses ab51320 at 1/100 dilution and anti-mouse IgG at 1/2500 as secondary.
![Overlay histogram showing HeLa cells stained with ab51320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26473_CDC5L-Primary-antibodies-ab51320-1.jpg)
Overlay histogram showing HeLa cells stained with ab51320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
Sousa MM, Zub KA, Aas PA, Hanssen-Bauer A, Demirovic A, Sarno A, Tian E, Liabakk NB, Slupphaug G. PLoS One. 2013;8(2):e55493.
Locus-specific proteomics by TChP: targeted chromatin purification. - Locus-specific proteomics by TChP: targeted chromatin purification.
Pourfarzad F, Aghajanirefah A, de Boer E, Ten Have S, Bryn van Dijk T, Kheradmandkia S, Stadhouders R, Thongjuea S, Soler E, Gillemans N, von Lindern M, Demmers J, Philipsen S, Grosveld F. Cell Rep. 2013 Aug 15;4(3):589-600.