Anti-Cdk6 antibody [EPR4515]

• Supplier: Abcam
• Catalog: ab124821
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4515
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in C-terminus of Human Cdk6
• Description: Rabbit Monoclonal
• Gene: CDK6
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab124821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124821, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilution

ab124821 at 1/100 dilution, staining Cdk6 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

ICC/IF image of ab124821 at 1/100 dilution, staining Cdk6 in HeLa cells.