Anti-CDKN2A/p14ARF antibody
| Name | Anti-CDKN2A/p14ARF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3642 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to the C-terminal region of Human p14ARF |
| Description | Rabbit Polyclonal |
| Gene | CDKN2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image was kindly supplied as part of the review submitted by Joost van Galen.
ICC/IF image of ab3642 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3642 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Dynamics of senescent cell formation and retention revealed by p14ARF induction - Dynamics of senescent cell formation and retention revealed by p14ARF induction
Tokarsky-Amiel R, Azazmeh N, Helman A, Stein Y, Hassan A, Maly A, Ben-Porath I. Cancer Res. 2013 May 1;73(9):2829-39.
Regulation of p14ARF expression by miR-24: a potential mechanism compromising the - Regulation of p14ARF expression by miR-24: a potential mechanism compromising the
To KH, Pajovic S, Gallie BL, Theriault BL. BMC Cancer. 2012 Feb 15;12:69.
Functional analyses of coronary artery disease associated variation on chromosome - Functional analyses of coronary artery disease associated variation on chromosome
Motterle A, Pu X, Wood H, Xiao Q, Gor S, Ng FL, Chan K, Cross F, Shohreh B, Poston RN, Tucker AT, Caulfield MJ, Ye S. Hum Mol Genet. 2012 Sep 15;21(18):4021-9.
Critical roles of DMP1 in human epidermal growth factor receptor 2/neu-Arf-p53 - Critical roles of DMP1 in human epidermal growth factor receptor 2/neu-Arf-p53
Taneja P, Maglic D, Kai F, Sugiyama T, Kendig RD, Frazier DP, Willingham MC, Inoue K. Cancer Res. 2010 Nov 15;70(22):9084-94.
The role of NPM, p14arf and MDM2 in precursors of bronchial squamous cell - The role of NPM, p14arf and MDM2 in precursors of bronchial squamous cell
Mascaux C, Bex F, Martin B, Burny A, Haller A, Paesmans M, Willard-Gallo K, Ninane V, Sculier JP. Eur Respir J. 2008 Sep;32(3):678-86.
Similar tumor suppressor gene alteration profiles in asbestos-induced murine and - Similar tumor suppressor gene alteration profiles in asbestos-induced murine and
Lecomte C, Andujar P, Renier A, Kheuang L, Abramowski V, Mellottee L, Fleury-Feith J, Zucman-Rossi J, Giovannini M, Jaurand MC. Cell Cycle. 2005 Dec;4(12):1862-9. Epub 2005 Dec 7.