Anti-CDKN2A/p19ARF antibody

• Supplier: Abcam
• Catalog: ab80
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ICC/IF ELISA IHC-P WB IHC-F
• Species Reactivities: Mouse
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse p19ARF
• Description: Rabbit Polyclonal
• Gene: Cdkn2a
• Conjugate: Unconjugated

Product images

All lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/mlLane 1 : 30 ug of mouse embryonic fibroblast total cell extract.Lane 2 : 30 ug of mouse embryonic fibroblast total cell extract.Lane 3 : 30 ug of mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.Performed under reducing conditions.

ab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+). Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the coverslips were incubated with ab80 at a concentration of 1ug/ml, diluted in PBS for 1 hour at room temperature. The antibody was then visualised using standard anti-rabbit secondary antibodies coupled to Texas Red.

ab80 at 1/300 staining mouse kidney (glomerulus) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

ab80 at 1/300 staining mouse brain cells by ICC/IF.Paraformaldehyde fixation, Triton X-100 permeabilization, followed by blocking with 3% BSA + 2% NGS for 1 hour at 20°C.Incubation with the primary antibody was carried out for 1 hour at 20°C.  The secondary antibody was an Alexa Fluor® conjugated Donkey anti-Rabbit polyclonal, diluted 1/400.See Abreview

ab80 staining CDKN2A/p19ARF in Mouse pancreatic neoplastic tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI.See Abreview

Product References

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