Anti-CEBP Beta antibody [A16]
| Name | Anti-CEBP Beta antibody [A16] |
|---|---|
| Supplier | Abcam |
| Catalog | ab18336 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | A16 |
| Applications | FC IHC-P IHC-F WB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein corresponding to Mouse CEBP Beta aa 1-268 |
| Description | Mouse Monoclonal |
| Gene | CEBPB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab18336 staining mouse microglia cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton prior to blocking in 5% serum for 30 minutes at 37°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor® 488 goat anti-mouse antibody (ab150113) diluted 1/500 was used as the secondary. The images show clear nuclear CEBP beta staining in microglial cells treated with LPS.See Abreview
ab18336 staining mouse hippocampus tissue sections by IHC-FoFr. Sections were PFA fixed and permeabilized with a commercially available Mouse on Mouse (MOM) kit (ab127055) prior to blocking with a commercially available blocking reagent for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 30 minutes at 25°C. A biotinylated rabbit anti-mouse antibody was used as the secondary.The animals were perfused with 4% paraformaldehyde solution, cryoprotected in sucrose, frozen, and 30 um coronal sections were obtained in a cryostat. Free floating sections were immersed for 5 min in 0.3% H2O2 to inactivate endogenous peroxidases, processed for immunohistochemistry with the MOM kit as indicated above and visualized using the avidin-biotin protocol ABC Peroxidase Staining kit and the diaminobenzidine method.Negative control tissue from CEBP beta KO mice was used.See Abreview
ab18336 staining CEBP in human normal skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 (ab64214) and blocking in 10% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 in PBT (PBS + 0.5% Tween-20 + 0.5% BSA) and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.See Abreview
 used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27372_CEBP-Beta-Primary-antibodies-ab18336-4.jpg)
Overlay histogram showing HT1080 cells stained with ab18336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18336, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Age-associated change of C/EBP family proteins causes severe liver injury and - Age-associated change of C/EBP family proteins causes severe liver injury and
Hong IH, Lewis K, Iakova P, Jin J, Sullivan E, Jawanmardi N, Timchenko L, Timchenko N. J Biol Chem. 2014 Jan 10;289(2):1106-18.
Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells. - Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells.
Dentesano G, Straccia M, Ejarque-Ortiz A, Tusell JM, Serratosa J, Saura J, Sola C. J Neuroinflammation. 2012 Jul 9;9:165.
Role of C/EBPbeta transcription factor in adult hippocampal neurogenesis. - Role of C/EBPbeta transcription factor in adult hippocampal neurogenesis.
Cortes-Canteli M, Aguilar-Morante D, Sanz-Sancristobal M, Megias D, Santos A, Perez-Castillo A. PLoS One. 2011;6(10):e24842.
CCAAT/enhancer binding protein beta deficiency provides cerebral protection - CCAAT/enhancer binding protein beta deficiency provides cerebral protection
Cortes-Canteli M, Luna-Medina R, Sanz-Sancristobal M, Alvarez-Barrientos A, Santos A, Perez-Castillo A. J Cell Sci. 2008 Apr 15;121(Pt 8):1224-34.