Anti-CENPC antibody [EPR15939]

• Supplier: Abcam
• Catalog: ab193666
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15939
• Applications: IP FC IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human CENPC aa 400-550
• Description: Rabbit Monoclonal
• Gene: CENPC
• Conjugate: Unconjugated

Product images

All lanes : Anti-CENPC antibody [EPR15939] (ab193666) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CENPC antibody [EPR15939] (ab193666) at 1/10000 dilution + K562 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CENPC antibody [EPR15939] (ab193666) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CENPC with ab193666 at 1/1000 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CENPC with ab193666 at 1/1000 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling CENPC with ab193666 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Western blot analysis of CENPC immunoprecipitated from Jurkat cell lysate using ab193666 at 1/100 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.