IHC image of CHI3L1 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86428, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Anti-CHI3L1 antibody [AT4A3] (ab86428) at 1/250 dilution + HepG2 cell lysates at 35 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/28274_CHI3L1-Primary-antibodies-ab86428-1.jpg)
Anti-CHI3L1 antibody [AT4A3] (ab86428) at 1/250 dilution + HepG2 cell lysates at 35 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique