IHC image of Choline Acetyltransferase staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70219, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI.
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI.