Anti-Claudin 3 antibody
| Name | Anti-Claudin 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15102 |
| Prices | $400.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Rat, Chicken, Human, Pig |
| Antigen | Synthetic peptide (Mouse) (C terminal) |
| Description | Rabbit Polyclonal |
| Gene | CLDN3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of PFA-fixed, paraffin embedded BALB/c murine ear tissue sections. Claudin 3 was stained, using ab15102 at 1/10 dilution, in the inner ear at the organ of Corti, Reissner’s membrane, periosteal lining of otic capsule, and the apical surface of the marginal cells of stria vascularis (upper panel) and in the middle ear intercellular junctions along lateral and basal cell membranes in the mucosal epithelium (lower panel). An AlexaFluor®488-conjugated IgG (1/50) was used as the secondary antibody.
Product References
Mouse middle ear ion homeostasis channels and intercellular junctions. - Mouse middle ear ion homeostasis channels and intercellular junctions.
Morris LM, DeGagne JM, Kempton JB, Hausman F, Trune DR. PLoS One. 2012;7(6):e39004.
Blood-testis barrier dynamics are regulated by testosterone and cytokines via - Blood-testis barrier dynamics are regulated by testosterone and cytokines via
Yan HH, Mruk DD, Lee WM, Cheng CY. FASEB J. 2008 Jun;22(6):1945-59.
Chronic metabolic acidosis upregulated claudin mRNA expression in the duodenal - Chronic metabolic acidosis upregulated claudin mRNA expression in the duodenal
Charoenphandhu N, Wongdee K, Tudpor K, Pandaranandaka J, Krishnamra N. Life Sci. 2007 Apr 17;80(19):1729-37. Epub 2007 Mar 2.