All lanes : Anti-Claudin 3 antibody (ab116165) at 1 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : LOVO (Human colon adenocarcinoma cell line) Whole Cell LysateLane 6 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222) with Immunizing peptide at 1 µg/mlLane 7 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051) with Immunizing peptide at 1 µg/mlLane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/mlLane 9 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/mlLane 10 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGo
IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.