Anti-Claudin 5 antibody [EPR7583]

• Supplier: Abcam
• Catalog: ab131259
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7583
• Applications: IHC-F WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues in the intracellular domain of Human Claudin 5
• Description: Rabbit Monoclonal
• Gene: CLDN5
• Conjugate: Unconjugated

Product images

Overlay histogram showing A549 cells stained with ab131259 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131259, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human lung squamous cell cancer lysateLane 3 : Human small cell lung cancer lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with ab131259 antibody at 1/100 dilution.

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with ab131259 antibody at 1/100 dilution.

ab131259 showing positive staining in Normal tonsil tissue.

ab131259 showing positive staining in Normal kidney tissue.

ab131259 showing positive staining in Normal placenta tissue.

ab131259 showing positive staining in Normal uterus tissue.

ab131259 showing positive staining in Squamous cell lung carcinoma tissue.

ab131259 showing positive staining in Normal colon tissue.