Anti-Claudin 7 antibody
| Name | Anti-Claudin 7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27487 |
| Prices | $398.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF IHC-P IHC-F ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: KAGYRAPRSYPKSNSSKEYV (Human) from the cytoplasmic C terminus of human Claudin 7 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | CLDN7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab27487, at a dilution of 1/200, staining Claudin 7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
ab27487 staining tissue sections of rat liver after oval cell activation. The sample was PFA fixed, permeabilized in 0,1% Triton X100 for 10 minutes and blocked with 2% serum, 2%BSA, 0.05% Tween 20 in PBS for 1 hour at room temperature. The primary antibody was diluted 1/100 and incubated for 12 hours at 4°C. A HRP conjugated donkey anti-rabbit was used as the secondary.See Abreview
ab27487 staining rat oval cells by ICC. Cells were fixed in ice cold methanol for 15 minutes and blocked in 3% serum for 1 hour at 25°C prior to incubation with ab27487 (diluted 1/50 in 3% Goat serum, 2% BSA, 0.01% Tween20) for 16 hours at 4°C. The secondary antibody was a Cy3® conjugated donkey anti-rabbit, diluted 1/100.See Abreview
All lanes : Anti-Claudin 7 antibody (ab27487) at 1/100 dilutionLane 1 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - Insoluble fraction (membrane)Lane 2 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - soluble fraction (cytoplasm)Lysates/proteins at 22 µg per lane.SecondaryAn HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL technique
Product References
Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive - Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive
Suren D, Yildirim M, Kaya V, Alikanoglu AS, Bulbuller N, Yildiz M, Sezer C. Med Sci Monit. 2014 Jul 20;20:1255-62.
Multimerization of adenovirus serotype 3 fiber knob domains is required for - Multimerization of adenovirus serotype 3 fiber knob domains is required for
Wang H, Li Z, Yumul R, Lara S, Hemminki A, Fender P, Lieber A. J Virol. 2011 Jul;85(13):6390-402.
A gene on the HER2 amplicon, C35, is an oncogene in breast cancer whose actions - A gene on the HER2 amplicon, C35, is an oncogene in breast cancer whose actions
Katz E, Dubois-Marshall S, Sims AH, Faratian D, Li J, Smith ES, Quinn JA, Edward M, Meehan RR, Evans EE, Langdon SP, Harrison DJ. Br J Cancer. 2010 Jul 27;103(3):401-10.
Expression of claudin7 is tightly associated with epithelial structures in - Expression of claudin7 is tightly associated with epithelial structures in
Kohno Y, Okamoto T, Ishibe T, Nagayama S, Shima Y, Nishijo K, Shibata KR, Fukiage K, Otsuka S, Uejima D, Araki N, Naka N, Nakashima Y, Aoyama T, Nakayama T, Nakamura T, Toguchida J. J Biol Chem. 2006 Dec 15;281(50):38941-50. Epub 2006 Oct 23.