Anti-Cleaved PARP antibody

• Supplier: Abcam
• Catalog: ab4830
• Prices: $392.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF WB
• Species Reactivities: Bovine, Human
• Antigen: Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP
• Description: Rabbit Polyclonal
• Gene: PARP1
• Conjugate: Unconjugated

Product images

Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1 µg/mL anti-PARP (pan) antibody or anti PARP cleavage site (214/215) specific antibody at 1 µg/mL. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-PARP cleavage site specific antibody only recognizes the 85 kDa fragment of PARP in apoptotic cells (lane 3) and does not react with full length PARP (lane 1). The PARP (pan) antibody confirms that nonapoptotic cells express full length PARP of 116 kDa (lane 2) and which is cleaved when apoptosis is induced (lane 4).

HeLa cells were induced into apoptosis with staurosporine at 0.5 µM for 5 hours (panel A) and were untreated as control (panel B). The cells were fixed in cold acetone for 5 minutes. Cells were incubated with the anti PARP cleavage site specific antibody (CCSA) at 10 µg/mL. Cells were then incubated with biotinylated goat anti-rabbit Igs followed by ABC and DAB. The data show that the anti-PARP CCSA specifically recognizes PARP in apoptotic cells. Taken together with Western blot data above, these data demonstrate the specificity of the anti-PARP CCSA for cleaved PARP.

All lanes : Anti-Cleaved PARP antibody (ab4830) at 1/1000 dilutionLane 1 : Non-induced Jurkat cellsLane 2 : Induced Jurkat cellsSecondaryGoat Anti-Rabbit HRPdeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Differential PKA activation and AKAP association determines cell fate in cancer - Differential PKA activation and AKAP association determines cell fate in cancer

Hedrick ED, Agarwal E, Leiphrakpam PD, Haferbier KL, Brattain MG, Chowdhury S. J Mol Signal. 2013 Oct 1;8(1):10.

Bomapin is a redox-sensitive nuclear serpin that affects responsiveness of - Bomapin is a redox-sensitive nuclear serpin that affects responsiveness of

Przygodzka P, Ramstedt B, Tengel T, Larsson G, Wilczynska M. BMC Cell Biol. 2010 Apr 30;11:30.

Cell death induced by N-methyl-N-nitrosourea, a model S(N)1 methylating agent, in - Cell death induced by N-methyl-N-nitrosourea, a model S(N)1 methylating agent, in

Koryllou A, Patrinou-Georgoula M, Troungos C, Pletsa V. Apoptosis. 2009 Sep;14(9):1121-33.

Forskolin protects keratinocytes from UVB-induced apoptosis and increases DNA - Forskolin protects keratinocytes from UVB-induced apoptosis and increases DNA

Passeron T, Namiki T, Passeron HJ, Le Pape E, Hearing VJ. J Invest Dermatol. 2009 Jan;129(1):162-6.

Abrogation of DNA vector-based RNAi during apoptosis in mammalian cells due to - Abrogation of DNA vector-based RNAi during apoptosis in mammalian cells due to

Ghodgaonkar MM, Shah RG, Kandan-Kulangara F, Affar EB, Qi HH, Wiemer E, Shah GM. Cell Death Differ. 2009 Jun;16(6):858-68.

p27 Kip1 nuclear localization and cyclin-dependent kinase inhibitory activity are - p27 Kip1 nuclear localization and cyclin-dependent kinase inhibitory activity are

Wang Q, Zhou Y, Wang X, Evers BM. Cell Death Differ. 2008 May;15(5):908-19.

Rapid and selective death of leukemia stem and progenitor cells induced by the - Rapid and selective death of leukemia stem and progenitor cells induced by the

Guzman ML, Li X, Corbett CA, Rossi RM, Bushnell T, Liesveld JL, Hebert J, Young F, Jordan CT. Blood. 2007 Dec 15;110(13):4436-44. Epub 2007 Sep 4.