Anti-Cleaved PARP antibody [E51]

Name	Anti-Cleaved PARP antibody [E51]
Supplier	Abcam
Catalog	ab32064
Prices	$403.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	E51
Applications	WB IHC-P
Species Reactivities	Mouse, Rat, Human, Hamster
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cleaved PARP aa 150-250
Description	Rabbit Monoclonal
Gene	Parp1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Cleaved PARP antibody [E51] (ab32064) at 1/1000 dilutionLane 1 : RAW264.7 cell lysateLane 2 : NIH/3T3 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Cleaved PARP antibody [E51] (ab32064) at 1/10000 dilutionLane 1 : Untreated Jurkat cell lysateLane 2 : Jurkat cell lysate treated with camptothecinLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32064 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded rat colon with purified ab32064 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

developed using the ECL techniquePerformed under reducing conditions.

Anti-Cleaved PARP antibody [E51] (ab32064) at 1/50000 dilution + MCF7 cell lysate at 100 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-Cleaved PARP antibody [E51] (ab32064) at 1/1000000 dilutionLane 1 : Jurkat cell lysate. Untreated.Lane 2 : Jurkat cell lysate. Treated with Camptothecin.

Immunohistochemical staining of paraffin embedded human breast carcinoma with unpurified ab32064 at a 1:100 dilution.

Product References

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