Anti-Collagen III antibody [Col-29]

• Supplier: Abcam
• Catalog: ab82354
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: Col-29
• Applications: IHC-P WB IHC-F
• Species Reactivities: Rat, Human, Mouse
• Antigen: Full length protein corresponding to Human Collagen III aa 1-1466
• Description: Mouse Monoclonal
• Gene: COL3A1
• Conjugate: Unconjugated

Product images

IHC image of Collagen III staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82354, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Collagen III antibody [Col-29] (ab82354)Lane 1 : HT1080 Whole cell lysateLane 2 : Human Colo320 Whole cell lysate