Anti-Collagen IV antibody
| Name | Anti-Collagen IV antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21295 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF ELISA RIA IHC-P |
| Species Reactivities | Human |
| Antigen | Full length native protein (purified): Collagen type IV extracted and purified from human placenta |
| Description | Rabbit Polyclonal |
| Gene | COL4A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Collagen IV staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21295, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Effects of serum-free culture at the air-liquid interface in a human - Effects of serum-free culture at the air-liquid interface in a human
Jean J, Bernard G, Duque-Fernandez A, Auger FA, Pouliot R. Tissue Eng Part A. 2011 Apr;17(7-8):877-88.
Gingival mucosa regeneration in athymic mice using in vitro engineered human oral - Gingival mucosa regeneration in athymic mice using in vitro engineered human oral
Rouabhia M, Allaire P. Biomaterials. 2010 Aug;31(22):5798-804.