All lanes : Anti-Contactin 1 antibody (ab74599) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-FoFr image of Contactin 1 (ab74599) staining on rat cerebral cortex sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview