Anti-CRM1 antibody
| Name | Anti-CRM1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24189 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human CRM1 |
| Blocking Peptide | Human CRM1 peptide |
| Description | Rabbit Polyclonal |
| Gene | XPO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-CRM1 antibody (ab24189) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab24189 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24189 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of CRM1 staining in Human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24189, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-CRM1 antibody (ab24189) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : HeLa nuclear lysateLane 3 : HeLa whole cell lysate with Human CRM1 peptide (ab25749) at 1 µg/mlLane 4 : HeLa nuclear cell lysate with Human CRM1 peptide (ab25749) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.
Product References
The myxobacterial metabolite ratjadone A inhibits HIV infection by blocking the - The myxobacterial metabolite ratjadone A inhibits HIV infection by blocking the
Fleta-Soriano E, Martinez JP, Hinkelmann B, Gerth K, Washausen P, Diez J, Frank R, Sasse F, Meyerhans A. Microb Cell Fact. 2014 Jan 29;13:17.
Evolution of a species-specific determinant within human CRM1 that regulates the - Evolution of a species-specific determinant within human CRM1 that regulates the
Sherer NM, Swanson CM, Hue S, Roberts RG, Bergeron JR, Malim MH. PLoS Pathog. 2011 Nov;7(11):e1002395.