Anti-CTGF antibody

• Supplier: Abcam
• Catalog: ab6992
• Prices: $400.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP ICC/IF ICC/IF WB IHC-P IHC-F IHC-F
• Species Reactivities: Mouse, Rat, Sheep, Human, Bovine
• Antigen: Recombinant fragment, corresponding to amino acids 223-348 of Mouse CTGF
• Description: Rabbit Polyclonal
• Gene: CTGF
• Conjugate: Unconjugated

Product images

ab6992 staining GTGF (green) in Human vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with mathacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/400) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Blue - DAPI Nuclei.See Abreview

ab6992 staining GTGF (green) in Mouse vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with mathacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/400) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Blue - DAPI Nuclei.See Abreview

ab6992 staining CTGF in Sheep articular cartilage tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% neutral-buffered formalin and blocked with 0.25% serum-free protein block for 10 minutes at room temperature. Samples were incubated with primary antibody (1/400) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.See Abreview

ab6992 at a 1/100 dilution staining CTGF from Mouse Tissue sections (Skin) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 12 hours and then detected using a Labeled AP polymer. The right side of image is regrown wounded epidermis and shows significant staining for CTGF.See Abreview

ab6992 at a 1/200 dilution staining CTGF in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated at +4°C for 9 hours. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).See Abreview

ab6992 staining CTGF in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview

ab6692 staining CTGF in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).See Abreview

All lanes : Anti-CTGF antibody (ab6992) at 1/1000 dilutionLane 1 : Extracellular matric within skeletal muscle from a mouse with fibrosis in the legsLane 2 : Human skeletal muscle whole tissue lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor® 488-conjugated goat anti-rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 36 kDa (why is the actual band size different from the predicted?)Exposure time : 2 minutesThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-CTGF antibody (ab6992) at 1/5000 dilution (in PBS-T for 9 hours at 4°C) + Mouse brain whole cell lysate at 50 µgSecondaryAn HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniqueObserved band size : 36 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 25°CSee Abreview

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