ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/mlLane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/mlLane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/mlLane 1 : Human brain cell lysate Lane 2 : Human brain cell lysate Lane 3 : Human brain cell lysate Lysates/proteins at 15 µg/ml per lane.SecondaryAnti-rabbit IgG HRP at 1/10000 dilution
ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC