![Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3804_ab185619-241616-wb-1.jpg)
Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
![Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3805_ab185619-241615-wb-2.jpg)
Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
![Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3806_ab185619-241614-wb-3.jpg)
Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
![All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3807_ab185619-241613-wb-4.jpg)
All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on spermatocytes of the Human testis is observed. Counter stained with Hematoxylin.Negative control obtained using PBS instead of ab185619 and secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human colon cancer labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasm staining on tumor cells of the Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.Negative control obtained using PBS instead of ab185619 and secondary antibody only.
Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: K562 whole cell extract 10 µg (Input).Lane 2: ab185619 IP in K562 whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds