Anti-Cyclin B1 antibody [V152]
| Name | Anti-Cyclin B1 antibody [V152] |
|---|---|
| Supplier | Abcam |
| Catalog | ab72 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | V152 |
| Applications | SDS-PAGE ICC/IF ICC/IF IHC-P FC WB IHC-F IHC-P IHC-F |
| Species Reactivities | Human, Mouse |
| Antigen | His-tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HCl and purified on Nickel beads |
| Description | Mouse Monoclonal |
| Gene | CCNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Cyclin B1 antibody [V152] (ab72) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951)Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3877_Cyclin-B1-Primary-antibodies-ab72-8.jpg)
All lanes : Anti-Cyclin B1 antibody [V152] (ab72) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951)Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab72 staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Product References
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Nabti I, Marangos P, Bormann J, Kudo NR, Carroll J. J Cell Biol. 2014 Mar 17;204(6):891-900.
Casein kinase 1delta-dependent Wee1 protein degradation. - Casein kinase 1delta-dependent Wee1 protein degradation.
Penas C, Ramachandran V, Simanski S, Lee C, Madoux F, Rahaim RJ, Chauhan R, Barnaby O, Schurer S, Hodder P, Steen J, Roush WR, Ayad NG. J Biol Chem. 2014 Jul 4;289(27):18893-903.
Human parvovirus B19 infection causes cell cycle arrest of human erythroid - Human parvovirus B19 infection causes cell cycle arrest of human erythroid
Luo Y, Kleiboeker S, Deng X, Qiu J. J Virol. 2013 Dec;87(23):12766-75.
The role of RING box protein 1 in mouse oocyte meiotic maturation. - The role of RING box protein 1 in mouse oocyte meiotic maturation.
Zhou L, Yang Y, Zhang J, Guo X, Bi Y, Li X, Zhang P, Zhang J, Lin M, Zhou Z, Shen R, Guo X, Huo R, Ling X, Sha J. PLoS One. 2013 Jul 11;8(7):e68964.
Tumor dynamics in response to antiangiogenic therapy with oral metronomic - Tumor dynamics in response to antiangiogenic therapy with oral metronomic
Kumar S, Mokhtari RB, Oliveira ID, Islam S, Toledo SR, Yeger H, Baruchel S. Transl Oncol. 2013 Aug 1;6(4):493-503. Print 2013 Aug.
The G protein coupled receptor 3 is involved in cAMP and cGMP signaling and - The G protein coupled receptor 3 is involved in cAMP and cGMP signaling and
Yang CR, Wei Y, Qi ST, Chen L, Zhang QH, Ma JY, Luo YB, Wang YP, Hou Y, Schatten H, Liu ZH, Sun QY. PLoS One. 2012;7(6):e38807.
Plk1 negatively regulates PRC1 to prevent premature midzone formation before - Plk1 negatively regulates PRC1 to prevent premature midzone formation before
Hu CK, Ozlu N, Coughlin M, Steen JJ, Mitchison TJ. Mol Biol Cell. 2012 Jul;23(14):2702-11.
APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle - APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle
Holt JE, Lane SI, Jennings P, Garcia-Higuera I, Moreno S, Jones KT. Mol Biol Cell. 2012 Oct;23(20):3970-81.
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Toledano Y, Zonis S, Ren SG, Wawrowsky K, Chesnokova V, Melmed S. Endocrinology. 2012 Oct;153(10):5011-22. Epub 2012 Jul 31.
MiR-145 modulates multiple components of the insulin-like growth factor pathway - MiR-145 modulates multiple components of the insulin-like growth factor pathway
Law PT, Ching AK, Chan AW, Wong QW, Wong CK, To KF, Wong N. Carcinogenesis. 2012 Jun;33(6):1134-41.