![Overlay histogram showing A549 cells stained with ab126828 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126828, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4809_ab126828-5-ab126828FC.jpg)
Overlay histogram showing A549 cells stained with ab126828 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126828, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab126828, at 1/200 dilution, labelling Cytochrome P450 1A1 in paraffin embedded Human ovarian cancer tissue by immunohistochemistry followed by DAB staining.
ab126828, at 1/200 dilution, labelling Cytochrome P450 1A1 in paraffin embedded Human rectum cancer tissue by immunohistochemistry followed by DAB staining.
![Anti-Cytochrome P450 1A1 antibody [6G5] (ab126828) at 1/500 dilution + Human Cytochrome P450 1A1 (aa 203-461) recombinant protein. This antibody has only been tested against recombinant protein. We have no data on the detection of endogenous protein.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4812_Cytochrome-P450-1A1-Primary-antibodies-ab126828-4.jpg)
Anti-Cytochrome P450 1A1 antibody [6G5] (ab126828) at 1/500 dilution + Human Cytochrome P450 1A1 (aa 203-461) recombinant protein. This antibody has only been tested against recombinant protein. We have no data on the detection of endogenous protein.