Anti-D4 GDI antibody [EPR14211(B)]

• Supplier: Abcam
• Catalog: ab181259
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14211(B)
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human D4 GDI aa 1-100
• Description: Rabbit Monoclonal
• Gene: ARHGDIB
• Conjugate: Unconjugated

Product images

Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. 

Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution + Human bone marrow lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

All lanes : Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : U937 cell lysateLane 3 : HL60 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution