Anti-DNA Ligase I antibody [10H5]
| Name | Anti-DNA Ligase I antibody [10H5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab615 |
| Prices | $401.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 10H5 |
| Applications | WB IP FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant human DNA Ligase I protein encoding amino acids 1-919 purified from baculovirus infected Sf9 cells |
| Description | Mouse Monoclonal |
| Gene | LIG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab615 at 1/150 staining normal human primary fibroblasts by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 16 hours. A FITC conjugated goat polyclonal antibody was used as the secondary.See Abreview
ab615 (2µg/ml) staining DNA Ligase 1 in human colonic mucosa, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of mucosal epithelium .Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![Overlay histogram showing Jurkat cells stained with ab615 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab615, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8928_DNA-Ligase-I-Primary-antibodies-ab615-2.jpg)
Overlay histogram showing Jurkat cells stained with ab615 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab615, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
A PCNA-derived cell permeable peptide selectively inhibits neuroblastoma cell - A PCNA-derived cell permeable peptide selectively inhibits neuroblastoma cell
Gu L, Smith S, Li C, Hickey RJ, Stark JM, Fields GB, Lang WH, Sandoval JA, Malkas LH. PLoS One. 2014 Apr 11;9(4):e94773.
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
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Okazaki fragment processing-independent role for human Dna2 enzyme during DNA - Okazaki fragment processing-independent role for human Dna2 enzyme during DNA
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Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in - Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in
Akbari M, Solvang-Garten K, Hanssen-Bauer A, Lieske NV, Pettersen HS, Pettersen GK, Wilson DM 3rd, Krokan HE, Otterlei M. DNA Repair (Amst). 2010 Jul 1;9(7):785-95.
Extracts of proliferating and non-proliferating human cells display different - Extracts of proliferating and non-proliferating human cells display different
Akbari M, Pena-Diaz J, Andersen S, Liabakk NB, Otterlei M, Krokan HE. DNA Repair (Amst). 2009 Jul 4;8(7):834-43.
The rate of base excision repair of uracil is controlled by the initiating - The rate of base excision repair of uracil is controlled by the initiating
Visnes T, Akbari M, Hagen L, Slupphaug G, Krokan HE. DNA Repair (Amst). 2008 Nov 1;7(11):1869-81.
Effect of polyamine deficiency on proteins involved in Okazaki fragment - Effect of polyamine deficiency on proteins involved in Okazaki fragment
Johansson VM, Miniotis MF, Hegardt C, Jonsson G, Staaf J, Berntsson PS, Oredsson SM, Alm K. Cell Biol Int. 2008 Dec;32(12):1467-77.
Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex - Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex
Muylaert I, Elias P. J Biol Chem. 2007 Apr 13;282(15):10865-72. Epub 2007 Feb 12.
Exchangeability of mammalian DNA ligases between base excision repair pathways. - Exchangeability of mammalian DNA ligases between base excision repair pathways.
Sleeth KM, Robson RL, Dianov GL. Biochemistry. 2004 Oct 12;43(40):12924-30.
Repair of single-strand DNA interruptions by redundant pathways and its - Repair of single-strand DNA interruptions by redundant pathways and its
Ho EL, Satoh MS. Nucleic Acids Res. 2003 Dec 1;31(23):7032-40.