All lanes : Anti-Dopamine D2 Receptor antibody (ab30743) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 7 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 8 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lysates/proteins at 10 µg per lane.SecondaryRabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab30743 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30743, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931 Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.