Anti-Dopamine Receptor D1 antibody [EP1560Y]
| Name | Anti-Dopamine Receptor D1 antibody [EP1560Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab81296 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1560Y |
| Applications | IHC-P FC WB |
| Species Reactivities | Mouse, Rat, Guinea Pig, Human |
| Antigen | Synthetic peptide corresponding to the C terminal regiom of human Dopamine Receptor D1 protein |
| Description | Rabbit Monoclonal |
| Gene | DRD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing SH-SY5Y cells stained with ab81296 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81296, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µgSecondaryHRP labelled goat anti rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9744_Dopamine-Receptor-D1-Primary-antibodies-ab81296-1.JPG)
Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µgSecondaryHRP labelled goat anti rabbit at 1/2000 dilution
Product References
Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in - Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in
Brewer KL, Baran CA, Whitfield BR, Jensen AM, Clemens S. Front Neural Circuits. 2014 Jun 11;8:62.
The dopamine D1 receptor is expressed and facilitates relaxation in airway smooth - The dopamine D1 receptor is expressed and facilitates relaxation in airway smooth
Mizuta K, Zhang Y, Xu D, Mizuta F, D'Ovidio F, Masaki E, Emala CW. Respir Res. 2013 Sep 2;14:89.