PathScan ® Total β-Actin Sandwich ELISA Kit
| Name | PathScan ® Total β-Actin Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7880 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse, Rat, Hamster, Monkey |
| Description | The PathScan ® Total β-Actin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of β-actin |
| Gene | ACTB |
| Supplier Page | Shop |
Product images
Figure 1. Treatment of HeLa cells with IFN-α stimulates phosphorylation of Stat3 at Tyr705 as detected by PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit #7300, but does not affect the level of β-actin as detected by PathScan ® Total β-Actin Sandwich ELISA Kit #7880. HeLa cells were treated with 100 ng/ml IFN-α for ten minutes at 37ºC before lysis. Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 (right panel) or Total β-Actin (13E5) Rabbit mAb #4970 (left panel) are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of the lysate from HeLa cells and the absorbance at 450 nm is shown.