A dilution series of Hela cell lysates (standard sample) in the working range of the assay.
Sample experiment showing FH levels in human liver homogenate (HLH), HepG2 cell lysates, HDFn cell lysates, MCF7 cell lysates and HeLa cell lysates, which is shown as a standard control sample. Cell lysates at varying concentrations within the working range of the assay were analyzed.
Sample experiment showing FH levels in human liver homogenate (HLH), HepG2 cell lysates, HDFn cell lysates, MCF7 cell lysates and HeLa cell lysates, which is shown as a standard control sample. The protein expression level in different samples were demonstrated relatively to Hela Standard
Figure 4. (Left) The FH Detector Antibody used in the ELISA kit immunocaptures FH from HepG2 Cell Lysate (lane 2), Human Liver Homogenate (lane 3), bovine Heart Homogenate (lane 4) and Rat Heart Homogenate (lane 5). Target has been confirmed by Mass Spectometric data. (Right) The antibody also works in immunocytochemistry and targets on Mitochondrial isozyme of FH.
Figure 3. (Left) The FH Capture Antibody used in the ELISA kit immunocaptures FH from Human Liver Homogenate (lane 2), bovine Heart Homogenate (lane 3) and HepG2 Cell Lysate (lane 4). Target has been confirmed by Mass Spectometric data. (Right) The antibody also works in immunocytochemistry and targets on Mitochondrial isozyme of FH.