Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.
Example standard curve of serially titrated Hela cell lysate in the working range of the assay.
Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane.
Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit.
Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines.
Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane.
Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green).
Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.