Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets)
| Name | Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) |
|---|---|
| Supplier | Abcam |
| Catalog | ab134005 |
| Category | Antibody Array |
| Prices | $275.00, $500.00, $720.00 |
| Sizes | 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes |
| Assay Type | Semi-quantitative |
| Sample Type | Tissue Extracts, Cell Lysate |
| Detection | Multiplex Protein Detectionmore details |
| Applications | Multiplex |
| Species Reactivities | Human |
| Supplier Page | Shop |
Product images
Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.
Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.