Human Obesity Antibody Array - Membrane (62 targets)
| Name | Human Obesity Antibody Array - Membrane (62 targets) |
|---|---|
| Supplier | Abcam |
| Catalog | ab169819 |
| Category | Antibody Array |
| Prices | $750.00, $1,100.00 |
| Sizes | 1 x 4 membranes, 1 x 8 membranes |
| Assay Type | Semi-quantitative |
| Sample Type | Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media |
| Detection | Multiplex Protein Detectionmore details |
| Applications | Multiplex |
| Species Reactivities | Human |
| Supplier Page | Shop |
Product images
5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.