IL-1 beta (Interleukin-1 beta) Human SimpleStep ELISA™ Kit
| Name | IL-1 beta (Interleukin-1 beta) Human SimpleStep ELISA™ Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab184861 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Cell culture supernatant, Serum, Plasma |
| Detection | Colorimetric |
| Sensitivity | 5.9 pg/ml |
| Range | 5 pg/ml - 5000 pg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | IL1B |
| Supplier Page | Shop |
Product images
Example of IL-1beta standard curve with the standard prepared in Sample Diluent NS.
Example of IL-1beta standard curve with the standard prepared in Sample Diluent 25BP. Background-subtracted data values (mean +/- SD) are graphed.
Titration of PBMC+PHA supernatant within the working range of the assay. Background subtracted data from duplicate measurements are plotted.
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous IL-1 beta levels.