IL-10 measured in culture supernatants from control (-) or activated cell lines, stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).
Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).
Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).
Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab100549
Representative standard curve using ab100549