Rabbit anti-Sp1 Antibody, Affinity Purified

Name	Rabbit anti-Sp1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0044
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Pig, Chicken, Dog, Amphibians, Goat, Bovine, Horse, Rabbit, Primate, Human, Mouse
Gene	SP1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0044</B>: Detection of Human SP1 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-SP1 (<B>Cat. No. PLA0044</B>) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0044: Detection of Human SP1 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-SP1 (Cat. No. PLA0044) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0044</B>: Detection of Human SP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), and 293T (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-SP1 Antibody <B>PLA0044</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0044: Detection of Human SP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), and 293T (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-SP1 Antibody PLA0044 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoblotting</B><BR/>Rabbit Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0044</B>: Detection of Mouse SP1 by Western Blot. Samples: Whole cell lysate from Renca (15 and 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-SP1 Antibody <B>PLA0044</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0044: Detection of Mouse SP1 by Western Blot. Samples: Whole cell lysate from Renca (15 and 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-SP1 Antibody PLA0044 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0044</B>: Detection of Human SP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-SP1 antibody <B>Cat. No. PLA0044</B> used for IP at 6 μg/mg lysate. SP1 was also immunoprecipitated by a SP1 Antibody B. For blotting immunoprecipitated SP1, <B>PLA0044</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Rabbit Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0044: Detection of Human SP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-SP1 antibody Cat. No. PLA0044 used for IP at 6 μg/mg lysate. SP1 was also immunoprecipitated by a SP1 Antibody B. For blotting immunoprecipitated SP1, PLA0044 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.