Rabbit anti-FOXO1a Antibody, Affinity Purified

Name	Rabbit anti-FOXO1a Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0084
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Rabbit, Rat, Pig, Horse, Primate, Bovine, Chimpanzee, Dog, Monkey, Human, Mouse
Gene	FOXO1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-FOXO1a Antibody, Affinity Purified: <B>Cat. No. PLA0084</B>: Detection of Human FOXO1a by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-FOXO1a (<B>Cat. No. PLA0084</B>) used at a dilution of 1:500 (2 μg/mL). Detection: DAB.

Immunohistochemistry
Rabbit Anti-FOXO1a Antibody, Affinity Purified: Cat. No. PLA0084: Detection of Human FOXO1a by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-FOXO1a (Cat. No. PLA0084) used at a dilution of 1:500 (2 μg/mL). Detection: DAB.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-FOXO1a Antibody, Affinity Purified: <B>Cat. No. PLA0084</B>: Detection of Human FOXO1a by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-FOXO1a Antibody <B>PLA0084</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-FOXO1a Antibody, Affinity Purified: Cat. No. PLA0084: Detection of Human FOXO1a by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-FOXO1a Antibody PLA0084 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-FOXO1a Antibody, Affinity Purified: <B>Cat. No. PLA0084</B>: Detection of Human FOXO1a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified Rabbit Anti-FOXO1a antibody <B>Cat. No. PLA0084</B> used for IP at 6 μg/mg lysate. FOXO1a was also immunoprecipitated by FOXO1a Antibody B. For blotting immunoprecipitated FOXO1a , <B>PLA0084</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Rabbit Anti-FOXO1a Antibody, Affinity Purified: Cat. No. PLA0084: Detection of Human FOXO1a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified Rabbit Anti-FOXO1a antibody Cat. No. PLA0084 used for IP at 6 μg/mg lysate. FOXO1a was also immunoprecipitated by FOXO1a Antibody B. For blotting immunoprecipitated FOXO1a , PLA0084 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.