Rabbit anti-SRC3 Antibody, Affinity Purified

Name	Rabbit anti-SRC3 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0095
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Chimpanzee, Primate, Human
Gene	NCOA3
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-SRC3 Antibody, Affinity Purified: <B>Cat. No. PLA0095</B>: Detection of SRC3 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 μg for WB, 10 mg for CoIP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-SRC3 (<B>Cat. No. PLA0095</B>) used at 0.33 μg/mL for WB and 20 μg/10 mg extract for CoIP. Detection: Alkaline phosphatase conjugated goat Anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 minutes for WB and Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3 for CoIP.

Immunoblotting
Rabbit Anti-SRC3 Antibody, Affinity Purified: Cat. No. PLA0095: Detection of SRC3 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 μg for WB, 10 mg for CoIP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-SRC3 (Cat. No. PLA0095) used at 0.33 μg/mL for WB and 20 μg/10 mg extract for CoIP. Detection: Alkaline phosphatase conjugated goat Anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 minutes for WB and Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3 for CoIP.