Goat anti-Sp1 Antibody, Affinity Purified

Name	Goat anti-Sp1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0307
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human, Mouse
Gene	SP1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Goat Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0307</B>: Detection of Mouse Sp1 by Immunohistochemistry. Sample: FFPE section of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (<B>Cat. No. PLA0307</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Mouse Sp1 by Immunohistochemistry. Sample: FFPE section of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (Cat. No. PLA0307) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunohistochemistry</B><BR/>Goat Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0307</B>: Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (<B>Cat. No. PLA0307</B>) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (Cat. No. PLA0307) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0307</B>: Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody <B>PLA0307</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody PLA0307 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoblotting</B><BR/>Goat Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0307</B>: Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody <B>PLA0307</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody PLA0307 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Goat Anti-Sp1 Antibody, Affinity Purified: <B>Cat. No. PLA0307</B>: Detection of Human Sp1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-Sp1 antibody <B>Cat. No. PLA0307</B> used for IP at 6 μg/mg lysate. Sp1 was also immunoprecipitated by Rabbit Anti-Sp1 antibody <B>Cat. No. PLA0044</B>. For blotting immunoprecipitated Sp1, <B>PLA0307</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-Sp1 antibody Cat. No. PLA0307 used for IP at 6 μg/mg lysate. Sp1 was also immunoprecipitated by Rabbit Anti-Sp1 antibody Cat. No. PLA0044. For blotting immunoprecipitated Sp1, PLA0307 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.