Rabbit anti-NIF1 Antibody, Affinity Purified

Name	Rabbit anti-NIF1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0145
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Monkey, Chimpanzee, Ape, Human, Primate, Horse, Guinea Pig, Marmoset
Gene	ZNF335
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-NIF1 Antibody, Affinity Purified: <B>Cat. No. PLA0145</B>: Detection of Human NIF1 by Immunohistochemistry. Sample: FFPE section of Human Hodgkin′s lymphoma. Antibody: Affinity purified Rabbit Anti-NIF1 (<B>Cat. No. PLA0145</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-NIF1 Antibody, Affinity Purified: Cat. No. PLA0145: Detection of Human NIF1 by Immunohistochemistry. Sample: FFPE section of Human Hodgkin′s lymphoma. Antibody: Affinity purified Rabbit Anti-NIF1 (Cat. No. PLA0145) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-NIF1 Antibody, Affinity Purified: <B>Cat. No. PLA0145</B>: Detection of Human and Mouse NIF1 by WB and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NIF1 Antibody (<B>Cat. No. PLA0145</B>) used for WB at 0.04 μg/mL (A) and at 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). NIF1 was also immunoprecipitated by Rabbit Anti-NIF1 Antibodies recognizing different epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunoblotting
Rabbit Anti-NIF1 Antibody, Affinity Purified: Cat. No. PLA0145: Detection of Human and Mouse NIF1 by WB and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NIF1 Antibody (Cat. No. PLA0145) used for WB at 0.04 μg/mL (A) and at 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). NIF1 was also immunoprecipitated by Rabbit Anti-NIF1 Antibodies recognizing different epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).